Scan a Plate on the Odyssey® F Imager

Once connected, click Multiwell Plate on the Choose Assay page to begin imaging gels.

The following imaging settings are used in this workflow. Other settings can be entered in the Custom workflow.

  • Resolution options:

    • 96-well plates: 84 µm, 169 µm
    • 384-well plates: 84 µm

  • Focus Offset: 1 – 5 mm

  • Well formats: 96 well, 384 well

  • Resolution options: 84 µm, 169 µm

  • Focus Offset: 1 – 5 mm

  • Well Formats: 6 well, 12 well, 24 well, 48 well, 96 well

Set Scan Area

Use the four buttons that correspond to the four locations on the Odyssey F plate holder to designate which plates you want to scan.

Choose detection options

  1. If you have multiple Scan Areas, click Edit Channels to choose detection options independently for each plate.

  2. Select the plate's well format.

  3. Click Scan to begin image acquisition.

Detection label reference

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Channel* Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 813 – 832 nm
700 IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ 5 685 nm 710 – 730 nm
520 CellTag™ 520 Stain, VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546 520 nm 570 – 610 nm
488 VRDye™ 490 Secondary Antibodies, FITC, Alexa Fluor 488 488 nm 538 – 552 nm

*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Scan

After clicking Scan on the Choose detection options page, the Scan page will open with the image acquisition process already started.

When the scan has completed, the Ready symbol will appear.

Stop Scan

If you need to stop an image acquisition before the acquisition process completes, click Stop Scan.

This will stop all pending Acquisitions and will remove any partially completed Acquisitions.

Export Acquisition

Ensure the Include all acquisitions option is selected to export every image for every channel from this acquisition process.

Export to Analyze

Click Export to Analyze to export images to a single file. Import the file into Empiria Studio® Software for analysis.

Export to Publish or Present

Click Export to Publish or Present to export image file(s). Images exported using this method are not intended for analysis.

Images can be exported as high resolution TIFF files (for journal publications) or as PNG files appropriate for digital presentation (such as a slide show).

When you are exporting a single image, the default export image size shown in the "Export" dialog is the largest dimension (i.e., width or height) of the image set to 10 cm (4 in), and the other dimension is set according to the aspect ratio of the image. If you change the width or height in the dialog, the other dimension will update automatically to maintain the correct aspect ratio.

See View Images in the Image Gallery for more information.

Workflow

  • Click Start New to return to the beginning of the workflow to acquire another gel image.
  • Click Rescan to acquire new image(s) with the same settings.
  • Click Home to disconnect from the imager and return to the Home page.