Scan a Membrane on the Odyssey® XF Imager or Odyssey Fc Imager

Once connected, click Membrane on the Choose Assay page to begin imaging chemiluminescent membranes.

Image settings for this workflow

  • Resolution: 125 µm

  • Chemi Acquisition Time Options: 30 sec, 1 min, 2 min (default), 5 min, 10 min

  • Chemi Manual Entry for Acquisition Time: 5 seconds to 60 minutes

    Click the button to enter a custom acquisition time from 5 seconds to 60 minutes.

  • Resolution: 125 µm

  • Fluorescence Acquisition Time Options: 30 sec, 1 min, 2 min (default), 5 min, 10 min

  • Manual Entry for Acquisition Time: 5 seconds to 60 minutes

Choose detection options

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 816 - 846 nm 600, 700, Chemi
700 IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680 685 nm 716 - 746 nm 600, 800, Chemi
Chemi HRP and AP chemiluminescent substrate NA NA 600, 700, 800
600 Custom 520 nm 573 - 637 nm 700, 800, Chemi

Scan

After clicking Scan on the Choose detection options page, the Scan page will open with the image acquisition process already started.

When the scan has completed, the Ready symbol will appear.

Stop Scan

If you need to stop an image acquisition before the acquisition process completes, click Stop Acquire.

This will stop all pending Acquisitions and will remove any partially completed Acquisitions.

Export Acquisition

Ensure the Include all acquisitions option is selected to export every image for every channel from this acquisition process.

Export to Analyze

Click Export to Analyze to export images to a single file. Import the file into Empiria Studio® Software for analysis.

Export to Publish or Present

Click Export to Publish or Present to export image file(s). Images exported using this method are not intended for analysis.

Images can be exported as high resolution TIFF files (for journal publications) or as PNG files appropriate for digital presentation (such as a slide show).

When you are exporting a single image, the default export image size shown in the "Export" dialog is the largest dimension (i.e., width or height) of the image set to 10 cm (4 in), and the other dimension is set according to the aspect ratio of the image. If you change the width or height in the dialog, the other dimension will update automatically to maintain the correct aspect ratio.

See View Images in the Image Gallery for more information.

Workflow

  • Click Start New to return to the beginning of the workflow to acquire another membrane image.
  • Click Repeat Acquire to acquire new image(s) with the same settings.
  • Click Home to disconnect from the imager and return to the Home page.