Welcome to the LI‑COR® Acquisition Software Help!
Custom Scan for the Odyssey® XF Imager or Odyssey Fc Imager
The following settings are available when using the Custom workflow. The Assay type of images acquired in this workflow will be "Other", which allows the images to be analyzed in more than membrane analysis in Empiria Studio® Software.
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Resolution Options: 125 µm
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Acquire times:
Click the button to enter a custom acquisition time from 5 seconds to 60 minutes.
Choose detection options
The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.
Channel | Labels | Excitation | Emission | Combined Selections Allowed |
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800 | IRDye® 800CW, DyLight 800 | 785 nm | 816 - 846 nm | 600, 700, Chemi |
700 | IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680 | 685 nm | 716 - 746 nm | 600, 800, Chemi |
Chemi | HRP and AP chemiluminescent substrate | NA | NA | 600, 700, 800 |
600 | SYBR Safe, Ethidium Bromide | 520 nm | 573 - 637 nm | 700, 800, Chemi |