Odyssey M Custom Scan

The following settings are available when using the Odyssey M Custom workflow.

  • Resolution Options: 5, 10, 20, 50 and 100 µm

    Scan area size can impact the available resolutions. For large scan areas, the resolution list on the Choose detection options page will include resolutions that will not produce a resultant image that exceeds the maximum size for the imaging system.

  • Focus Offset: -1.00 – 5.00 mm

  • Chemi membrane acquisition times: 30 sec, 1 min, 2 min (default), 5 min, 10 min, 20 min, 60 min

Choose detection options

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Channel Example Detection Labels Excitation Emission Combined Selections Allowed
800 IRDye 800CW, DyLight 800 785 nm 816 - 840 nm 700, 520, 488, RGB Epi, RGB Trans
700 IRDye 680LT, IRDye 680RD, IRDye 700, Revert 700 Total Protein Stain, Alexa Fluor 680, CellTag 700 Stain, DRAQ5 685 nm 721 - 740 nm 800, 520, 488, RGB Epi, RGB Trans
520 Revert 520 Total Protein Stain, CellTag 520 Stain, VRDye™ 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP) 520 nm 570 - 610 nm 800, 700, 488, RGB Epi, RGB Trans
520A DRAQ5 520 nm 721 - 740 nm 800, 700, 488, RGB Trans
520B Custom 520 nm 816 - 840 nm 800, 700, 488, RGB Trans
488 VRDye 490, FITC, Green Fluorescent Protein (GFP) 488 nm 519 - 543 nm 800, 700, 520, RGB Epi, RGB Trans
488A SYPRO Ruby, SYPRO Orange 488 nm 570 - 610 nm 800, 700, 520, RGB Epi
488B StarBright Blue 700 488 nm 721 - 740 nm 800, 700, 520, RGB Epi
488C Custom 488 nm 816 - 840 nm 800, 700, 520, RGB Epi
Chemi HRP and AP chemiluminescent substrates NA NA 700, 520, RGB Epi
RGB Epi Colorimetric Protein MW Markers, Ponceau S NA NA 800, 700, 520, 488, Chemi, or 630 Epi, 525 Epi, 470 Epi
630 Epi Pierce Reversible Protein Stain, TMB (blotting) 631 - 659 nm Clear Window RGB Epi
525 Epi Ponceau S, NBT/BCIP 500 - 530 nm Clear Window RGB Epi
470 Epi Custom 447 - 472 nm Clear Window RGB Epi
RGB Trans H&E Stain, Alcian Blue, Coomassie stain, Silver stain NA NA 800, 700, 520, 520A, 488
630 Trans Coomassie stain, TMB ELISA (non- stopped) 631 - 659 nm Clear Window None
525 Trans Custom 500 - 530 nm Clear Window None
470 Trans Silver stain, TMB – stopped ELISA, OPD 447 - 472 nm Clear Window None