Shapes Group

Shapes can be drawn on the image using the Draw Ellipse or Draw Freehand tools in the Shapes group. The Shape will be drawn in all channels that are displayed. Select the channel(s) to display and the display color in the Display tab on the right.

If one channel is displayed in pseudo color, the other channel must be gray scale with white shapes on black background.

• To draw an ellipse, click the Draw Ellipse tool. Drag from the center of the area of fluorescence on the image to one corner.
• To draw a freehand shape, click the Draw Freehand tool. Drag around the area of fluorescence on the image.
• Click Select to return to selection mode.

The Trim tool removes part of a Freehand or Auto shape to improve the fit of the shape to the area of fluorescence. To use the Trim tool:

1. Select one channel to be displayed and disable the other channel in the Display tab on the right.
2. Select a drawn shape by clicking on it in selection mode. The outline of the shape will change to a dotted line.
3. Click the Trim tool. Drag a line through the selected shape. The line must intersect the shape in two places.
4. Click on one side of the line to remove that side of the shape.