Quantification of Total Protein in a Lane

Signal

Sum of the individual pixel intensity values (Total) for a lane minus the product of the background value and area.

Signal = Total - (Background x Area)

Average Signal

Average Signal for Lanes in Empiria Studio

Background

Empiria Studio 1.3 automatically evaluates the local area around each band box or lane box to determine the background value. Read this section for more information about how Adaptive Background Subtraction works.

If the computed background value is extremely small, a "0" will appear in the Analysis Table in the Background column. To see all the digits of the computed background value, export the Analysis Table to a CSV file or to an Excel spreadsheet.

St. Dev.

Standard deviation of lane signals within a replicate group.

n = The number of data points

x_i = Each of the values of the data

x̅ = The mean of the x_i

% CV

Coefficient of variation of signals for lanes within a replicate group.

Percent CV Calculation

SNR

The signal-to-noise ratio (SNR) compares the signal in a lane to the background noise calculated for the lane. See Signal-to-Noise Ratio for more information.

Avg. SNR

Average signal-to-noise ratio for lanes within a replicate group.