Calculation Definitions

• Area: Number of pixels enclosed by a lane box or band box.

• Signal: Sum of the individual pixel intensity values (Total) for a band box (or lane box) minus the product of the background value and area.

  Signal = Total - (Background x Area)

• Background: See Adaptive Background Subtraction for more information.

• Pixel Intensity: A value proportional to the amount of light collected from a single pixel. You can adjust the overall brightness of an image, but pixel intensities remains the same.

• Total: Sum of the individual pixel intensities enclosed by a band box or lane box.