Using the Profiles Tab to Inspect a Lane Profile

When a lane from a Western or MPX^™ Western analysis is selected, the pixel intensity profile throughout the lane can be seen in the Profiles tab.

If Image Studio Lane is selected as the background subtraction method in the Define Type list , a line representing lane background can be displayed beneath the lane profile (see below)(see A lane profile displayed in the Profiles tab is shown with background displayed and background subtracted.).

• To display the lane background line, select the Display Lane Background check box and a light colored line representing the background will appear beneath the original lane profile.
• To display the lane profile with background subtracted, select the Subtract Lane Background check box and the graph will be redrawn with lane background subtracted. The signal has already been calculated with the background subtracted, even if Subtract Lane Background has not been selected.